Catalog Number - 68322-15P-4

68322-15P-4 Cogent RP PFP™ HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for complex separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using ultra‑high purity silica.

The column chemistry provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it particularly effective for halogenated, aromatic, and polar analytes. It also serves as a suitable alternate column for USP methods where PFP selectivity is advantageous.

With a 3 µm particle size, this column delivers higher efficiency than 5 µm columns of the same phase, producing sharper peaks and improved resolution. As expected, the smaller particle size results in increased backpressure compared to larger particle columns.

150 mm Length – Maximum Resolution for Complex Separations

The 150 mm length is ideal for methods requiring maximum resolving power and improved separation of closely related compounds.

• Higher resolution than shorter column lengths

• Improved separation of structurally similar analytes

• Increased peak capacity for complex mixtures

✅ Recommended for analytical methods requiring high resolution without moving to preparative-scale columns

Flow Rate & System Requirements (4.0 mm ID, 3 µm)

⚠️ Compatible with standard analytical HPLC systems, with moderate to higher backpressure

• Typical flow rate range: ~0.7 – 1.3 mL/min

• Higher efficiency compared to 5 µm columns

• Increased backpressure due to smaller particle size

• Suitable for UV and standard analytical detectors

✅ Best Practice: Filter samples using a 0.2 µm syringe filter prior to injection to maintain column performance

Why Choose PFP (F5) Columns for Selective Separations

Enhanced Retention for Halogenated Compounds

• Strong interaction with fluorinated and halogenated analytes

• Improved selectivity vs C18 columns

Multiple Interaction Mechanisms

• π‑π interactions for aromatic compounds

• Dipole and hydrogen bonding interactions

• Greater flexibility for method development

Versatile 100 Å Pore Structure

• Suitable for small to moderately sized molecules

• Balanced performance across applications

Cogent RP PFP™ Column Advantages

Fully End‑Capped Surface

• Minimizes silanol activity

• Improves peak symmetry

High Efficiency (3 µm Particles)

• More efficient than 5 µm columns

• Sharper peaks and improved resolution

High Purity Silica

• Consistent and reproducible chromatographic performance

• Long column lifetime

Typical Applications

• Halogenated compound analysis

• Aromatic compound separations

• Polar compound retention

• Pharmaceutical QA/QC workflows

• Method development and optimization

• USP alternate methods

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Designed for standard analytical HPLC systems (4.0 mm ID)

• Higher backpressure vs larger particle columns

• Use 0.2 µm filtration prior to injection

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.